Archaea: Methods and Protocols (Methods in Molecular Biology)

Description: 1. Archaea: a goldmine for molecular biologists and evolutionists Patrick Forterre Part I: Genetic manipulation of Archaea 2. Progress and challenges in archaeal genetic manipulation Catherine Harrison and Thorsten Allers 3. Genetic manipulation of Haloferax species Ambika Dattani, Catherine Harrison, and Thorsten Allers 4. CRISPR interference as a tool to Repress Gene Expression in Haloferax volcanii Thandi S. Schwarz, Sandra S. Schreiber, and Anita Marchfelder 5. Transformation Techniques for the Anaerobic Hyperthermophile Thermococcus kodakarensis Geraldy L. S. Liman, Meghan E. Stettler and Thomas J. Santangelo 6. Genetic Methods and Construction of Chromosomal Mutations in Methanogenic Archaea Johanna Thomsen, Katrin Weidenbach, William W. Metcalf and Ruth A. Schmitz 7. An interdomain conjugation protocol for plasmid-DNA transfer into Methanothermobacter thermautotrophicus ¿H Christian Fink, Largus T. Angenen, and Bastian Molitor 8. Methods for Markerless Gene Deletion and Plasmid-Based Expression in Sulfolobus acidocaldarius Xing Ye, Alejandra Recalde, Sonja-Verena Albers and Marleen van Wolferen 9. A Rapid Targeted Gene Inactivation Approach in Sulfolobus islandicus Changyi Zhang, Serina M. Taluja, Emily N. Hallett, and Rachel J. Whitaker 10. Transposon Insertion Mutagenesis in hyperthermophilic crenarchaeon Sulfolobus islandicus Changyi Zhang and Rachel J. Whitaker 11. Reprogramming CRISPR-mediated RNA interference for silencing of essential genes in Sulfolobales Erika Wimmer, Isabelle Anna Zink and Christa Schleper Part II: Molecular Biology of Archaea 12. Progress and challenges in archaeal molecular biology Finn Werner 13. ChIP-seq Occupancy Mapping of the Archaeal Transcription Machinery Fabian Blombach, Kathy L. Smollett, and Finn Werner 14. Conversion of ribosome-protected mRNA to DNA and deep sequencing for ribosome profiling in Haloferax volcanii Diego Rivera Gelsinger, Emma Dallon, and Jocelyne DiRuggiero 15. Small RNA-sequencing library preparation for the halophilic archaeon Haloferax volcanii Diego Rivera Gelsinger and Jocelyne DiRuggiero 16. Quantitative Mass Spectrometry by SILAC in Haloferax volcanii - Ricardo L. Couto-Rodriguez, Daniel Gal, Lana J. McMillan, Jin Koh, Sixue Chen, and Julie A. Maupin-Furlow 17. Proteome turnover analysis in Haloferax volcanii by a heavy isotope multilabelling approach Roberto A. Paggi, Stefan P. Albaum, Ansgar Poetsch, and Micaela Cerletti 18. Proteomic sample preparation and data analysis in line with the Archaeal Proteome Stefan Schulze and Mechthild Pohlschroder. 19. In vivo protein crosslinking and co-immunoprecipitation in Haloferax volcanii Roberto A. Paggi, Rosana E. De Castro and Micaela Cerletti 20. Proteolytic activity assays in haloarchaea Roberto Alejandro Paggi, María Inés. Giménez, and Rosana Esther De Castro 21. Carotenoids from Haloarchaea: Extraction, fractionation and characterization María Sandra Churio, Micaela Cerletti, and Rosana Esther De Castro 22. Metal nanoparticles biosynthesis using the halophilic archaeon Haloferax volcanii Mariana Inés Costa and María Inés Giménez 23. Simplified enzymatic synthesis of 2-keto-3-deoxy-D-gluconate from D-gluconate using the gluconate dehydratase from Thermoproteus tenax Svenja Höfmann, Promise Aku Dziwornu, Thomas Klaus, Thomas Knura, Roland Wohlgemuth, Christopher Bräsen, and Bettina Siebers PART III: Cellular Biology of Archaea 24. Progress and Challenges in Archaeal Cellular Biology Analysis Marleen van Wolferen and Sonja-Verena Albers 25. Methods to Analyze Motility in Eury- And Crenarchaea Megha Patro, Marleen van Wolferen, Xing Ye, Sonja-Verena Albers, and Tessa E.F. Quax 26. Immersed Liquid Biofilm and Honeycomb Pattern Formations in Haloferax volcanii Zuha Mutan, Heather Schiller, Stefan Schulze, and Mechthild Pohlschroder 27. Cost-effective and Versatile Analysis of Archaeal Surface Adhesion Under Shaking and Standing Conditions Stefan Schulze, Heather Schiller, Zuha Mutan, Jordan Solomonic, Orkan Telhan, and Mechthild Pohlschroder 28. Cell Adhesion and Biofilm Formation Analysis Débora Nercessian and Juan Pablo Busalmen 29. BrdU incorporation and labelling of nascent DNA to Investigate Archaeal Replication using super-resolution imaging Roxane Lestini, Yoann Collien, Debora Olivier, Nicolas Olivier, and Hannu Myllykallio 30. Isolation, Purification, and Characterization of Membrane Vesicles from Haloarchaea Joshua Mills and Susanne Erdmann 31. Archaeal Viruses: Production of Virus Particles and Vesicle-Like Viruses and Purification Using Asymmetrical Flow Field-Flow Fractionation Katri Eskelin and Hanna M. Oksanen Part IV: Environmental Biology 32. Progress and Challenges in Studying the Ecophysiology of Archaea Panagiotis S. Adam, Till L. V. Bornemann, and Alexander J. Probst 33. Reconstruction of Archaeal Genomes Assembly from Short-Read Metagenomes Till L. V. Bornemann, Panagiotis S. Adam, and Alexander J. Probst Part V: Outreach protocol 34. Outreach: Accessible and Insightful Scientific Learning Experiences Using the Microorganism Haloferax volcanii Heather Schiller, Criston Young, Stefan Schulze, and Mechthild Pohlschroder

Price: 323 AUD

Location: Hillsdale, NSW

End Time: 2024-12-05T18:26:14.000Z

Shipping Cost: 26.4 AUD