Protein NMR: Methods and Protocols (Methods in Molecular Biology) [Paperback]

Description: This volume covers state-of-the-art applications of solid-state and solution nuclear magnetic resonance( NMR) spectroscopy to study protein structure, dynamics and interactions. Chapters detail various aspects of data acquisition and processing, determination of the structure, multi-timescale dynamics of entities ranging from individual proteins to large macromolecular complexes to intact viral assemblies. The final two chapters will highlight the promise of NMR beyond field strengths of 1 GHz to study the structure, dynamics and interactions of a larger class of proteins and protein complexes of extraordinary biological interest. Written in the highly successful Methods in Molecular Biology series format, chapters provide detailed laboratory protocols and troubleshooting tips that would be of great practical help to NMR spectroscopists with different levels of expertise. Authoritative and cutting-edge, Protein NMR: Methods and Protocol aims to ensure successful results in the further study of this vital field. 1. NMR of Macromolecular Assemblies and Machines at 1 GHz and Beyond: New Transformative Opportunities for Molecular Structural Biology Caitlin M. Quinn, Mingzhang Wang, and Tatyana Polenova 2. Experimental Aspects of Polarization Optimized Experiments (POE) for Magic Angle Spinning Solid-state NMR of Microcrystalline and Membrane-Bound Proteins T. Gopinath and Gianluigi Veglia 3. Afterglow Solid-State NMR Spectroscopy Gili Abramov and Nathaniel J. Traaseth 4. Filamentous Bacteriophage Viruses: Preparation, Magic-angle Spinning Solid-state NMR Experiments and Structure Determination Omry Morag, Nikolaos G. Sgourakis, Gili Abramov, and Amir Goldbourt 5. Spherical Nanoparticle Supported Lipid Bilayers: A Tool for Modeling Protein Interactions with Curved Membranes Erin R. Tyndall and Fang Tian 6. Rapid Prediction of Multi-dimensional NMR Data Sets using FANDAS Siddarth Narasimhan, Deni Mance, Cecilia Pinto, Markus Weingarth, Alexandre M. J. J. Bonvin, and Marc Baldus 7. Strategies for Efficient Sample Preparation for Dynamic Nuclear Polarization Solid State NMR of Biological Macromolecules Boris Itin and Ivan V. Sergeyev 8. In-vitro Dissolution Dynamic Nuclear Polarization for Sensitivity Enhancement of NMR with Biological Molecules Yaewon Kim, Yunyi Wang, Hsueh-Ying Chen, and Christian Hilty 9. Determination of Protein ps-ns Motions by High-Resolution Relaxometry Samuel F. Cousin, Pavel Kaderávek, Nicolas Bolik-Coulon, and Fabien Ferrage 10. Characterizing Protein Dynamics with NMR R1 r Relaxation ExperimentsFrancesca Massi and Jeffrey W. Peng 11. CPMG Experiments for Protein Minor Conformer Structure Determination Anusha B Gopalan, D Flemming Hansen, and Pramodh Vallurupalli 12. Probing the Atomic Structure of Transient Protein Contacts by Paramagnetic Relaxation Enhancement Solution NMR Vincenzo Venditti and Nicolas L. Fawzi 13. From Raw Data to Protein Backbone Chemical Shifts Using NMRFx Processing and NMRViewJ Analysis Bruce A. Johnson 14. Protein Structure Elucidation from NMR Data with the Program Xplor-NIH Guillermo A. Bermejo and Charles D. Schwieters 15. Practical Nonuniform Sampling and Non-Fourier Spectral Reconstruction for Multidimensional NMR Mark W. Maciejewski, Adam D. Schuyler, and Jeffrey C. Hoch 16. Covariance NMR Processing and Analysis for Protein Assignment Bradley J. Harden and Dominique P. Frueh 17. Structures of Dynamic Protein Complexes: Hybrid Techniques to Study MAP Kinase Complexes and the ESCRT System Wolfgang Peti, Rebecca Page, Evzen Boura, and Bartosz Rozycki 18. Implementation of the NMR CHEmical Shift Covariance Analysis (CHESCA): A Chemical Biologist's Approach to Allostery Stephen Boulton, Rajeevan Selvaratnam, Rashik Ahmed, and Giuseppe Melacini 19. High-efficiency Expression of Yeast-derived G Protein-coupled Receptors and 19F Labeling for Dynamical Studies Libin Ye, Alexander P. Orazietti, Aditya Pandey, and R. Scott Prosser 20. Quantitative Determination of Interacting Protein Surfaces in Prokaryotes and Eukaryotes by Using In-cell NMR Spectroscopy David S. Burz, Christopher M. DeMott, Asma Aldousary, Stephen Danserea, and Alexander Shekhtman

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